The development of a PCR-based marker linked to resistance to the blackcurrant gall mite (Cecidophyopsis ribis Acari: Eriophyidae).

摘要: Gall mite (Cecidophyopsis ribis) is the most serious pest of blackcurrant (Ribes nigrum L.), causing damaging condition known as ‘big bud’ and also transmitting reversion virus (BRV) within between plantations. The identification resistant germplasm at present a time-consuming expensive process, dependent on field infestation plots. Resistance based gene Ce introgressed from gooseberry has been used in UK breeding programmes for blackcurrant. Using bulked segregant analysis, 90 AFLP primer combinations were screened linkage map constructed around resistance locus controlled by Ce. Sixteen produced fragment progeny gall mite-resistant parent, but not susceptible parent; subsequent testing individual identified an closely linked to resistance. This fragment, designated E41M88-280, was converted PCR-based marker sequence-specific primers, amplifying only individuals. Validation this across range with different genetic backgrounds confirmed its reliability containing conversion sequence-based PCR simplifies application therefore increases utility selection high-throughput