Conversion of AFLP fragments tightly linked to SCMV resistance genes Scmv1 and Scmv2 into simple PCR-based markers.
作者： C. Dussle , M. Quint , M. Xu , A. Melchinger , T. Lübberstedt
DOI: 10.1007/S00122-002-0964-7
关键词:
摘要: In a previous study, bulked segregant analysis with amplified fragment length polymorphisms (AFLPs) identified several markers closely linked to the sugarcane mosaic virus resistance genes Scmv1 on chromosome 6 and Scmv2 3. Six AFLP (E33M61-2, E33M52, E38M51, E82M57, E84M59 E93M53) were located 3 two (E33M61-1 E35M62-1) 6. Our objective in present study was sequence respective bands order convert these dominant into more simple reliable polymerase chain reaction (PCR)-based sequence-tagged site markers. resulted either complete identical sequences between six inbreds investigated this or revealed single nucleotide within inbred lines were, therefore, not converted. One marker (E35M62-1) converted an insertion/deletion (indel) second (E33M61-2) cleaved polymorphic marker. Mapping of both PCR-based confirmed their localization same region (E33M61-2 3; E35M62-1 6) as original Thus, will be useful for marker-assisted selection facilitate map-based cloning SCMV genes.
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