Identification and Mapping of a Novel Sugarcane Mosaic Virus Resistance Gene in Maize

作者: De-Gui ZHANG , Ye-Hong LIANG , Li BAI , Shi-Huang ZHANG , Zhang-Ying XI

DOI: 10.1016/S1875-2780(09)60002-X

关键词: Molecular markerGeneSugarcane mosaic virusPopulationGermplasmGene mappingMaize dwarf mosaic virusBiologyGeneticsBulked segregant analysis

摘要: Abstract Sugarcane mosaic virus (SCMV) causes considerable damage to maize (Zea mays L.) in China. Characterization of resistance gene(s) serves as the basis for effective selection breeding programs. The objective this study was identify novel genes conferring SCMV from germplasm A total 21 BC2F3 populations derived 8 intercross combinations were artificially inoculated with under field conditions. numbers resistant and susceptible plants population Ye 478 × Hai 9-21 consistent theoretical 1:3 phenotypic ratio, suggesting that a recessive gene parent responsible SCMV. Bulked segregant analysis (BSA) simple sequence repeat (SSR) marker used verify designated scm3. Gene scm3 located bin 3.04–3.05, flanked by SSR markers umc1965 bnlg420 genetic distances 45.7 6.5 cM, respectively. Four additional linked detected, which associated order umc1965–scm3–bnlg420–umc1307–umc2265–bnlg2241–umc2166.

参考文章(35)
E. J. Dollinger, W. R. Findley, L. E. Williams, Resistance of Inheritance to Maize Dwarf Mosaic Virus in Maize (Zea mays L.)1 Crop Science. ,vol. 10, pp. 412- 415 ,(1970) , 10.2135/CROPSCI1970.0011183X001000040029X
S. Sanz-Alferez, T. E. Richter, S. H. Hulbert, J. L. Bennetzen, The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles. Theoretical and Applied Genetics. ,vol. 91, pp. 25- 32 ,(1995) , 10.1007/BF00220854
B. Naidu, L. M. Josephson, Genetic Analysis of Resistance to the Corn Virus Disease Complex 1 Crop Science. ,vol. 16, pp. 167- 172 ,(1976) , 10.2135/CROPSCI1976.0011183X001600020001X
K. D. Simcox, M. D. McMullen, R. Louie, Co-segregation of the maize dwarf mosaic virus resistance gene, Mdm1, with the nucleolus organizer region in maize. Theoretical and Applied Genetics. ,vol. 90, pp. 341- 346 ,(1995) , 10.1007/BF00221975
D. M. Bubeck, M. M. Goodman, W. D. Beavis, D. Grant, Quantitative trait loci controlling resistance to gray leaf spot in maize. Crop Science. ,vol. 33, pp. 838- 847 ,(1993) , 10.2135/CROPSCI1993.0011183X003300040041X
M. L. Xu, A. E. Melchinger, X. C. Xia, T. Lübberstedt, High-resolution mapping of loci conferring resistance to sugarcane mosaic virus in maize using RFLP, SSR, and AFLP markers. Molecular Genetics and Genomics. ,vol. 261, pp. 574- 581 ,(1999) , 10.1007/S004380051003
Jian-yu Wu, Jun-qiang Ding, Yan-xiu Du, Yan-bo Xu, Xue-cai Zhang, Genetic analysis and molecular mapping of two dominant complementary genes determining resistance to sugarcane mosaic virus in maize Euphytica. ,vol. 156, pp. 355- 364 ,(2007) , 10.1007/S10681-007-9384-8
P. J. Loesch, M. S. Zuber, Inheritance of Resistance to Maize Dwarf Mosaic Virus 1 Crop Science. ,vol. 12, pp. 350- 352 ,(1972) , 10.2135/CROPSCI1972.0011183X001200030027X
C. Dussle, M. Quint, M. Xu, A. Melchinger, T. Lübberstedt, Conversion of AFLP fragments tightly linked to SCMV resistance genes Scmv1 and Scmv2 into simple PCR-based markers. Theoretical and Applied Genetics. ,vol. 105, pp. 1190- 1195 ,(2002) , 10.1007/S00122-002-0964-7
A. E. Melchinger, L. Kuntze, R. K. Gumber, T. Lübberstedt, E. Fuchs, Genetic basis of resistance to sugarcane mosaic virus in European maize germplasm Theoretical and Applied Genetics. ,vol. 96, pp. 1151- 1161 ,(1998) , 10.1007/S001220050851