作者: De-Gui ZHANG , Ye-Hong LIANG , Li BAI , Shi-Huang ZHANG , Zhang-Ying XI
DOI: 10.1016/S1875-2780(09)60002-X
关键词: Molecular marker 、 Gene 、 Sugarcane mosaic virus 、 Population 、 Germplasm 、 Gene mapping 、 Maize dwarf mosaic virus 、 Biology 、 Genetics 、 Bulked segregant analysis
摘要: Abstract Sugarcane mosaic virus (SCMV) causes considerable damage to maize (Zea mays L.) in China. Characterization of resistance gene(s) serves as the basis for effective selection breeding programs. The objective this study was identify novel genes conferring SCMV from germplasm A total 21 BC2F3 populations derived 8 intercross combinations were artificially inoculated with under field conditions. numbers resistant and susceptible plants population Ye 478 × Hai 9-21 consistent theoretical 1:3 phenotypic ratio, suggesting that a recessive gene parent responsible SCMV. Bulked segregant analysis (BSA) simple sequence repeat (SSR) marker used verify designated scm3. Gene scm3 located bin 3.04–3.05, flanked by SSR markers umc1965 bnlg420 genetic distances 45.7 6.5 cM, respectively. Four additional linked detected, which associated order umc1965–scm3–bnlg420–umc1307–umc2265–bnlg2241–umc2166.