The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles.

摘要: The Rp3 locus of maize conditions race-specific resistance to a fungal rust pathogen, Puccinia sorghi. Both morphological and DNA markers were employed characterize alleles accurately position on the genetic map. marker polymorphisms distinctive each allele identified, allowing identification specific in cases where races that differentiate particular are not available. In population 427 progeny, Rg1 found be completely linked, while Lg3 was approximately 3 cM proximal long arm chromosome 3. this same population, 12 RFLP mapped relative Rp3; closest UMC102 (about 1cM distal Rp1) NPI114 (1–2 proximal). These additional probes used nature extent flanking carried along when six different backcrossed into single background. Depending upon investigated, minimum 2–10cM polymorphic retained through introgression process. addition, many map near detect an fragment region, indicating portions chromosomal segment have been tendemly duplicated. materials results generated will permit marker-assisted entry backgrounds lay foundation for eventual map-based cloning Rp3.