Microtubules become more dynamic but not shorter during preprophase band formation: a possible "search-and-capture" mechanism for microtubule translocation.

摘要: The dynamic behavior of the microtubule cytoskeleton plays a crucial role in cellular organization, but physical mechanisms underlying (re)organization plant cells are poorly understood. We investigated dynamics tobacco BY-2 suspension during interphase and formation preprophase band (PPB), cytoskeletal structure that defines site cytokinesis. Here we show after 2 h accumulation PPB concurrent disappearance elsewhere cortex, is completed starts to breakdown exponentially already 20 min before onset prometaphase. During PPB, instability, i.e., stochastic alternating between growing shrinking phases, cortical microtubules outside increases significantly, do not become shorter. Based on this, as well cross-linking lack evidence for motor involvement, propose "search-and-capture" mechanism formation, which regulation instability causes more possibly longer, while activity developing preferentially stabilizes these "searching" microtubules. Thus, gradually disappear from cortex aggregate forming PPB. Cell Motil. Cytoskeleton 57:246-258, 2004. (C) 2004 Wiley-Liss, Inc.