Egg yolk IgY: protection against rotavirus induced diarrhea and modulatory effect on the systemic and mucosal antibody responses in newborn calves.

摘要: Bovine rotavirus (BRV) is an important cause of diarrhea in newborn calves. Local passive immunity the most efficient protective strategy to control disease. IgY technology (the use chicken egg yolk immunoglobulins) economic and practical alternative prevent BRV dairy The aim this study was evaluate protection immunomodulation induced by oral administration enriched specific experimentally infected All calves groups Gp 1, 2 3 received colostrum (CC; virus neutralization Ab titer - VN=65,536; ELISA IgG(1)=16,384) prior gut closure. After closure, milk supplemented with 6% BRV-immune [(Gp 1) VN=2048; titer=4096] or non-immune 2) VN<4; titer<4] twice a day, for 14 days. Calves receiving CC only deprived (CD) fed antibody (Ab) free served as controls (Gp 4, respectively). were inoculated 10(5.85)focus forming units (FFU) virulent IND at days age. Control 4) developed severe diarrhea. Around 80% 1 (IgY 4096) infected, but they showed (4/5) against RV-specific detected feces indicating that avian antibodies (Abs) remained intact after passage through gastrointestinal tract. At post infection day 21, duodenum major site secreting cells (ASC) all experimental groups. Mucosal ASC responses isotypes significantly higher treated groups, independently specificity treatment, components modulated immune response mucosal level. These results indicate supplementing calves' diets first life BRV-specific represents promising Moreover strong active intestinal mucosa following yolk, regardless treatment.