Three-Dimensional Reconstruction from Serial Sections

作者: Randle W. Ware , Vincent Lopresti

DOI: 10.1016/S0074-7696(08)60956-0

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摘要: Publisher Summary This chapter reviews some of the methods three-dimensional reconstruction that have been successfully used in study many different types tissue. Historically, its first applications were embryology, where it enabled workers to see systematically for time spatial relationships developing organs and systems. Its application medicine biology has permitted an understanding both normal abnormal tissues which was impossible with ordinary histological techniques conjunction single-section analysis. With advent electron microscope, describes morphology smallest things resolvable by instrument. Since is one few making a detailed examination fine structure, must be hoped proper systematization can progress from modeling objects observed under 100 sections morphological analysis, whatever means, those nonmolecular intercellular are responsible integrative capacities system cells.Finally, we describe newer attemptsat being developed specifically investigationof nervous

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