Deletion of Polyamine Transport Protein PotD Exacerbates Virulence in Glaesserella (Haemophilus) parasuis in the Form of Non-biofilm-generated Bacteria in a Murine Acute Infection Model.

摘要: Polyamines are small, polycationic molecules with a hydrocarbon backbone and multiple amino groups required for optimal cell growth. The potD gene, belonging to the ABC (ATP-binding cassette) transport system potABCD, encodes bacterial substrate-binding subunit of polyamine system, playing pivotal role in metabolism swine pathogen Glaesserella parasuis possesses an intact pot operon, studies presented here mainly examined involvement PotD pathogenesis. A potD-deficient mutant was constructed using virulent G. strain SC1401 by natural transformation; immuno-electron microscopy used identify subcellular location native protein; electron microscope adopted inspect biofilm morphology; immunofluorescence technique employed study cellular adhesion, levels inflammation apoptosis. TSA++-pre-cultured showed significantly reduced adhesion capacity PK-15 MLE-12 cells. Likewise, we also found attenuation virulence murine models focusing on clinical sign, H&E, IFA However, when grown TSB++, recovered normal levels, along high level radical oxygen species formation host. expression could actively stimulate production ROS Raw 264.7. Our data suggested that from has binding potential polyamine, is essential full within mouse models. highly dependent its TSA++ culture conditions rather than biofilm-formation.