Development of an indirect ELISA assay for the detection of IgG antibodies against the ORF1 of Torque teno sus viruses 1 and 2 in conventional pigs.
作者： David Nieto , Laura Martínez-Guinó , Alexandra Jiménez-Melsió , Joaquim Segalés , Tuija Kekarainen
DOI: 10.1016/J.VETMIC.2015.08.023
关键词:
摘要: Torque teno sus viruses (TTSuV, family Anelloviridae) cause long lasting and persistent infection in pigs under subclinical scenarios, are potentially linked to several economically important swine diseases. Currently, little is known about immune response against TTSuV infections. In this study, an ELISA assay was developed based on the ORF1-A recombinant protein of two TTSuVs, namely TTSuV1 (genus Iotatorquevirus) TTSuV2 Kappatorquevirus). The used study development humoral longitudinally sampled clinically healthy their dams. Anti IgG found serum sows for both TTSuVs. From 15 sows, (100%) 13 (83%) had anti TTSuV2, respectively. Pig sero-prevalences at first sampling (4 weeks age) were 65% (24/37) 5% (2/37) For TTSuV1, highest prevalence observed 21 25, with 68% (25/37) sero-positive pigs. Quantitative PCR (qPCR) results week revealed that 26 out 32 (81%) positive TTSuV1. case 21, 84% (31/37) being sero-positive. At same week, 92% (34/37) qPCR positive. summary, IgGs detected piglets different ages, indicating these animals could mount a However, high percentage viremic presence suggests antibodies not able remove TTSuVs from circulation.
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