Molecular characterization of the ORF2 of Torque teno sus virus 1a and Torque teno sus virus 1b detected in cases of postweaning multisystemic wasting syndrome in Mexico.

摘要: Worldwide Torque teno sus virus (TTSuV, genus Iotatorquevirus) species have been regarded as possible agents associated with porcine circovirus-associated disease. Iotatorquevirus possess high genomic variability, suggesting that diverse genotypes are widely geographically distributed. In this study, we validated the variability of Iotaroquevirus in pigs postweaned multisystemic wasting syndrome. Genomic DNA from nine TTSuV1a-positive tissues and 15 TTSuV1b-positive was used to amplify complete ORF2 each by nested PCR perform a molecular characterization. It found Mexican TTSuV1a sequences belong genotype B, sharing phylogenetic origin, nucleic acid amino sequence similarity dominant epitope conformation commercially linked countries, such United States, Canada China, whereas TTSuV1b A, being more divergent among other displaying low nucleotide identity worldwide A sequences. both species, PTPase-like signature motif identified predicted sequence, conserved for than sequences, which several substitutions were observed. These changes may influence epitopes different arrays determined genotypes. account pathogenic differences modifying viral replication immune response, depicted human TTV.