Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2.
作者： Luis G. Giménez-Lirola , Priscilla F. Gerber , Raymond R. Rowland , Patrick G. Halbur , Yao-Wei Huang
DOI: 10.1016/J.RVSC.2014.02.014
关键词: Antibody detection 、 Vaccination 、 Virology 、 Serum samples 、 Torque teno sus virus 、 Immunoassay 、 Seroprevalence 、 Antibody 、 Biology 、 Porcine reproductive and respiratory syndrome virus 、 Immunology
摘要: A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome type (PRRSV-1) PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve exposed to TTSuV2 on day 0, 20/20 vaccinated with a vaccine 35, 63. Anti-TTSuV detected in 30% the pigs 90% by 35. All had detectable anti-PRRSV-2 21 days after vaccination. Field 17 farms also tested. The seroprevalence both PRRSV TTSuV increased age. Comparison FMIA an ELISA revealed good correlation young but high rate false positives older Cross-reaction between types problem.
ed.ac.uk
sci-hub.se 参考文章(37)
Cavanagh D, Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Archives of Virology. ,vol. 142, pp. 629- 633 ,(1997)
T. Loula, Mystery pig disease. Agri-Practice (USA). ,vol. 12, pp. 23- 34 ,(1991)
E A Nelson, J Christopher-Hennings, T Drew, G Wensvoort, J E Collins, D A Benfield, Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. Journal of Clinical Microbiology. ,vol. 31, pp. 3184- 3189 ,(1993) , 10.1128/JCM.31.12.3184-3189.1993
T. Kekarainen, J. Segalés, Torque teno sus virus in pigs: an emerging pathogen? Transboundary and Emerging Diseases. ,vol. 59, pp. 103- 108 ,(2012) , 10.1111/J.1865-1682.2011.01289.X
Y.-W. Huang, K. K. Harrall, B. A. Dryman, T. Opriessnig, E. M. Vaughn, M. B. Roof, X.-J. Meng, Serological profile of Torque teno sus virus species 1 (TTSuV1) in pigs and antigenic relationships between two TTSuV1 genotypes (1a and 1b), between two species (TTSuV1 and 2), and between porcine and human anelloviruses Journal of Virology. ,vol. 86, pp. 10628- 10639 ,(2012) , 10.1128/JVI.00176-12
Susan Pang, Jodie Smith, David Onley, Julie Reeve, Michael Walker, Carole Foy, A comparability study of the emerging protein array platforms with established ELISA procedures. Journal of Immunological Methods. ,vol. 302, pp. 1- 12 ,(2005) , 10.1016/J.JIM.2005.04.007
M. Aramouni, J. Martínez, D. Nieto, T. Kekarainen, J. Segalés, Exploratory study of Torque teno sus viruses in pulmonary inflammatory lesions in pigs. Veterinary Microbiology. ,vol. 162, pp. 338- 344 ,(2013) , 10.1016/J.VETMIC.2012.09.011
Y.W. Huang, Y.Y. Ni, B.A. Dryman, X.J. Meng, Multiple infection of porcine Torque teno virus in a single pig and characterization of the full-length genomic sequences of four U.S. prototype PTTV strains: Implication for genotyping of PTTV Virology. ,vol. 396, pp. 289- 297 ,(2010) , 10.1016/J.VIROL.2009.10.031
S. Dea, C. A. Gagnon, H. Mardassi, B. Pirzadeh, D. Rogan, Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: comparison of the North American and European isolates. Archives of Virology. ,vol. 145, pp. 659- 688 ,(2000) , 10.1007/S007050050662
Sherry A. Dunbar, Applications of Luminex® xMAP™ technology for rapid, high-throughput multiplexed nucleic acid detection Clinica Chimica Acta. ,vol. 363, pp. 71- 82 ,(2006) , 10.1016/J.CCCN.2005.06.023