Hydrogen peroxide release from mouse peritoneal macrophages: dependence on sequential activation and triggering.

摘要: Using a specific and sensitive fluorometric assay, the H2O2 release from as few 2 X 10(5) mouse peritoneal macrophages could be detected continuously quantitated. It is emphasized that assay measured release, not production. Induction of required sequential application two stimuli: administration an activating agent to mice 4 days 10 wk before all harvest, exposure cells in vitro triggering agent. BCG was most effective agent, resulting which triggered almost copiously (8 nmol/10(6) per 5 min) PMN (9 NMOL/10(6) min). Casein C. parvum also serve activators, but thioglycollate FCS were ineffective after single injections. PMA potent maximal rate latency about 40 s for suspension. Other agents included ionophore A23187, concanavalin A presence cytochalasin B, phagocytosis. attributed cell suspensions or preparations adherent cells, lymphocytes. Indirect evidence suggested formed superoxide anion. These observations appear justify renewed interest idea may important macrohpage antimicrobial antitumor mechanisms.