Terminal-phosphate-labeled nucleotides with new linkers

作者: Paul Mitsis , Mark McDougall , John MacKlin , Anup Sood , Carl Fuller

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摘要: The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are detecting nucleic acid sample, based on use as substrates for polymerases, salt. Further provided complexes well with new linkers enhanced properties.

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