Identification of the glycosidase inhibitors swainsonine and calystegine B2 in Weir vine (Ipomoea sp. Q6 [aff. calobra]) and correlation with toxicity.

摘要: The polyhydroxy alkaloid glycosidase inhibitors swainsonine [1] and calystegine B2 [6] have been identified as constituents of the seeds Australian plant Ipomoea sp. Q6 [aff. calobra] (Weir vine) by gas chromatography-mass spectrometry their biological activity specific glycosidases. This plant, which is known only from a small area southern Queensland, has reported to produce neurological disorder when consumed livestock. extract showed inhibition alpha-mannosidase, beta-glucosidase, alpha-galactosidase, consistent with presence 1 alkaloids class. Histological examination brain tissue field cases sheep cattle poisoned Weir vine lesions similar those observed in animals swainsonine-containing poison peas (Swainsona spp.) Australia locoweeds (Astragalus Oxytropis North America. These results indicate that poisoning an additional manifestation induced lysosomal storage disease, mannosidosis, possibly exacerbated enzymes beta-glucosidase alpha-galactosidase B2. first example single species capable producing structurally distinct inhibitors, namely, indolizidine nortropane classes.