Plasma proteomic profile associated with platelet dysfunction after trauma.

摘要: Background Coagulopathic bleeding is a major cause of mortality after trauma, and platelet dysfunction contributes to this problem. The causes are relatively unknown, but great deal can be learned from the plasma environment about possible pathways involved. Objective Describe changes in proteomic profile associated with trauma. Methods Citrated blood was collected severely injured trauma patients at time their arrival Emergency Department. Samples were 110 patients, subset twenty-four identified by preserved (n=12) or impaired aggregation response five different agonists. Untargeted proteomics performed nanoflow liquid chromatography tandem mass spectrometry. Protein abundance levels for each patient normalized total protein concentration control hemodilution crystalloid fluid infusion prior draw. Results Patients more otherwise demographically similar those retained function. Of 232 proteins detected, twelve significantly between groups. These fall into several broad categories related function, including microvascular obstruction activation, immune protease activation. Conclusions This observational study provides description change identifies most profound changes. involving these salient targets immediate investigation better understand identify intervention.