A DNA-binding fraction of mouse kidney 3-ketosteroid reductase: Comparison with androgen receptors

摘要: Abstract Since approximately 1% of 3-ketosteroid reductase (which metabolizes dihydrotestosterone [17β-hydroxy-5α-androstan-3-one] to 5α-androstane-3α,17β-diol or 5α-androstane-3α,17β-diol) from mouse kidney cytosol adheres DNA under conditions that allow virtually complete androgen receptor binding, these two DNA-binding activities were compared in extracts and hypothalamus-preoptic area. This fraction was distinguished several ways: (1) its pattern elution DNA-cellulose with steps increasing NaC1 concentration differed for receptors wild-type kidney; (2) it influenced differently by the mutation Tfm , both level pattern; (3) relatively stable at moderate (25°C) temperatures which rapidly inactivated ligand-free same cytosols; (4) not proportional levels between tissues, area kidney. By criteria, a simple relationship activity is unlikely.