Lead induces increased water permeability in astrocytes expressing aquaporin 4

摘要: The water channel aquaporin 4 (AQP4) is abundantly expressed in astrocytes. There now compelling evidence that AQP4 may contribute to an unfavorable course brain edema. Acute lead intoxication a condition causes damage preceded by Here we report increases permeability (P-f) A rat astrocyte cell line does not express was transiently transfected with tagged green fluorescent protein (GFP). Using confocal laser scanning microscopy measured these cells and AQP4-negative located on the same plate. AQP4-expressing astrocytes had three-fold higher than expressing AQP4. Lead exposure induced significant, 40%, increase AQP4, but no effect Pf persisted after washout, while treatment chelator, meso-2,3-dimercaptosuccinic acid, abolished lead-induced Pf. of attenuated presence calcium (Ca2+)/ calmodulin-dependent kinase 11 (CaMKII) inhibitor, C inhibitor. In where consensus site for CaMKII phosphorylation mutated, failed permeability. also increased astroglial primary culture, which endogenous 3. situ hybridization studies oral intake three days showed change distribution mRNA. It suggested lead-triggered stimulation transport pathology acute intoxication.