Immunotargeting of the Pulmonary Endothelium via Angiotensin-Converting-Enzyme in Isolated Ventilated and Perfused Human Lung

摘要: Vascular immunotargeting of catalase via angiotensin-converting-enzyme (ACE) attenuated lung ischemia reperfusion injury in the rat. As this might be a promising modality for extension viability human grafts transplantation we tested hypothesis whether anti-ACE antibodies are suitable protection within model isolated perfused and ventilated resections. Right after surgery cancer, specimens were isolated, under physiological conditions with 500 μg either mouse monoclonal (mAb) to ACE (9B9, I2H5, 3G8) or non-immune IgG (as negative control) followed by wash-out perfusion. Perfusion pressure, pH weight gain measured before during After mAb perfusion period tissue was uptake mAbs immunohistochemistry enzyme-capture technique. Furthermore, antibody concentration shedding perfusate. We found that activity tumor normal did not differ between groups different mAbs. However, (17.0 ± 6.0 U/g) significantly higher compared (6.0 3.0; p < 0.01). Absolute retaining 1.3 1.1% injected dose per gram tissue, 0.7 0.7% (0.1 0.1%/g; Anti-ACE perfusate dropped 47 11% (p 0.001) at 40 min No significant difference depletion from has been observed. 9B9 showed most intense immunostaining (i.e., uptake) each experiment i2H5 3G8 These results validate possibility pulmonary endothelium vivo conditions. useful investigate targeted therapies cancer without side effects patient.