Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

作者: Fabrice Gielen , Raphaelle Hours , Stephane Emond , Martin Fischlechner , Ursula Schell

DOI: 10.1073/PNAS.1606927113

关键词:

摘要: … sorting of droplets and the corresponding droplet sorting … droplet frequency at which stable and reliable sorting was … The faster the droplets moved through the sorting junction, the …

参考文章(54)
Z. Huang, P.-C. Maness, N. Dowe, A. Mohagheghi, M. Newman, J. McMillan, Rapid detection of Zymomonas mobilis redox activity using 5-cyano-2,3-tolyl-tetrazolium chloride (CTC). BioTechniques. ,vol. 29, pp. 424- 428 ,(2000) , 10.2144/00293BM04
Kihwan Choi, Jared M. Mudrik, Aaron R. Wheeler, A guiding light: spectroscopy on digital microfluidic devices using in-plane optical fibre waveguides Analytical and Bioanalytical Chemistry. ,vol. 407, pp. 7467- 7475 ,(2015) , 10.1007/S00216-015-8913-X
Pierre-Yves Colin, Anastasia Zinchenko, Florian Hollfelder, Enzyme engineering in biomimetic compartments. Current Opinion in Structural Biology. ,vol. 33, pp. 42- 51 ,(2015) , 10.1016/J.SBI.2015.06.001
Kunishige Kataoka, Harumi Takada, Tohru Yoshimura, Setsuo Furuyoshi, Nobuyoshi Esaki, Toshihisa Ohshima, Kenji Soda, Site-directed mutagenesis of a hexapeptide segment involved in substrate recognition of phenylalanine dehydrogenase from Thermoactinomyces intermedius Journal of Biochemistry. ,vol. 114, pp. 69- 75 ,(1993) , 10.1093/OXFORDJOURNALS.JBCHEM.A124142
Vijay Srinivasan, Vamsee K. Pamula, Richard B. Fair, An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids Lab on a Chip. ,vol. 4, pp. 310- 315 ,(2004) , 10.1039/B403341H
Nicholas J Turner, Directed evolution drives the next generation of biocatalysts Nature Chemical Biology. ,vol. 5, pp. 567- 573 ,(2009) , 10.1038/NCHEMBIO.203
Enrico Mastrobattista, Valerie Taly, Estelle Chanudet, Patrick Treacy, Bernard T Kelly, Andrew D Griffiths, None, High-Throughput Screening of Enzyme Libraries: In Vitro Evolution of a β-Galactosidase by Fluorescence-Activated Sorting of Double Emulsions Chemistry & Biology. ,vol. 12, pp. 1291- 1300 ,(2005) , 10.1016/J.CHEMBIOL.2005.09.016
Huimin Zhao, Lori Giver, Zhixin Shao, Joseph A. Affholter, Frances H. Arnold, Molecular evolution by staggered extension process (StEP) in vitro recombination Nature Biotechnology. ,vol. 16, pp. 258- 261 ,(1998) , 10.1038/NBT0398-258
U. T. Bornscheuer, G. W. Huisman, R. J. Kazlauskas, S. Lutz, J. C. Moore, K. Robins, Engineering the third wave of biocatalysis Nature. ,vol. 485, pp. 185- 194 ,(2012) , 10.1038/NATURE11117
Balint Kintses, Christopher Hein, Mark F. Mohamed, Martin Fischlechner, Fabienne Courtois, Céline Lainé, Florian Hollfelder, Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution Chemistry & Biology. ,vol. 19, pp. 1001- 1009 ,(2012) , 10.1016/J.CHEMBIOL.2012.06.009