Bone formation and bone resorption in failed total joint arthroplasties: histomorphometric analysis with histochemical and immunohistochemical technique.

摘要: To elucidate the reactions of bone around aseptically loosened total joint arthroplasties, 24 interface tissues with adjacent were obtained in 17 revision operations (11 hips and six knees). The morphology surface next to membrane was investigated histochemical immunohistochemical techniques then histomorphometrically analysed. One-third surface. 32.69 +/- 5.16% (mean SE) (n = 24), showed positive alkaline phosphatase activity. contact cells for CD11b (a macrophage marker) amounted 19.33 24). proportion osteoclastic resorption estimated by vitronectin receptor expression 7.67 1.82% 21). Tissues retrieved from sites where radiographic evidence osteolysis present 12) had a significantly larger extent CD11b-positive than did areas without 12, p 0.0067, Mann-Whitney U test), whereas no significant difference observed resorption. These data demonstrate that active formation, regarded as repair process, is most common feature even revised cases. They also highlight role played macrophages, not producing inflammatory mediators could activate osteoclasts, but primarily responsible loss osteolytic lesions.