Concentration of Lipocalin Region of Collagen XXVII Alpha 1 in the Serum of Dogs with Hemangiosarcoma

摘要: Background: Hemangiosarcoma (HSA) is a common malignancy of dogs with characteristic early, aggressive metastasis. Diagnosis HSA challenging because lack sensitive and specific diagnostic tests. Hypothesis: Specific proteins that are increased in serum might represent useful biomarkers the disease. Animals: Thirty-four 42 healthy from Ontario Veterinary College Teaching Hospital. Methods: This case-control study compared dogs. Proteins were separated by 2-dimensional difference gel electrophoresis identified liquid chromatography tandem mass spectrometry. Results: Western blot analysis showed collagen XXVII peptide concentration large metastatic burdens (1,488, 231–3,754 DU; median, minimum-maximum); was, on average, 9.5-fold higher than (156; 46–2,101 DU). While concentrations for osteosarcomas (678; 124–3,251 DU), lymphomas (423; 92–2,777 DU), carcinomas (1,022; 177–3,448 DU), inflammatory disease also increased, values consistently lower those HSA. Receiver operating curves revealed an estimated area under curve 83% cases whereas areas other neoplastic nonneoplastic diseases nondiscriminatory. Serum before splenectomy (1,350; 1,156–1,929 DU) was reduced after tumor removal (529; 452–562 DU) chemotherapy but 2 recurrence (511–945 DU; 493–650 DU). Conclusions Clinical Importance: Collagen be diagnosis monitoring advanced