Transmucosal macromolecular drug delivery.

摘要: Mucosal surfaces are the most common and convenient routes for delivering drugs to body. However, macromolecular such as peptides proteins unable overcome mucosal barriers and/or degraded before reaching blood stream. Among approaches explored so far in order optimize transport of these macromolecules across barriers, use nanoparticulate carriers represents a challenging but promising strategy. The present paper aims compare characteristics potential nanostructures based on mucoadhesive polysaccharide chitosan (CS). These CS nanoparticles, CS-coated oil nanodroplets (nanocapsules) lipid nanoparticles. behavior nanoparticles already reported [A. Vila, A. Sanchez, M. Tobio, P. Calvo, M.J. Alonso, Design biodegradable particles protein delivery, J. Control. Rel. 78 (2002) 15-24; R. Fernandez-Urrusuno, C. Remunan-Lopez, J.L. Vila-Jato, Enhancement nasal absorption insulin using Pharm. Res. 16 (1999) 1576-1581; Garcia-Fuentes, D. Torres, New surface-modified delivery vehicles salmon calcitonin (submitted publication).] compared with those nanocapsules originally here. three types systems have size nanometer range positive zeta that was attributed presence their surface. They showed an important capacity association insulin, proteins, tetanus toxoid. Their mechanism interaction epithelia investigated Caco-2 model cell line. results caused concentration-dependent reduction transepithelial resistance monolayer. Moreover, within concentrations investigated, were internalized monolayer manner. This uptake slightly enhanced by coating but, previously published [M. Prego, Triglyceride-chitosan oral delivery: evaluation vivo publication)], highly dependent nature core. Nevertheless, differences (solid core or oily core) cells did not consequence behaviour. Indeed, both (nanocapsules nanoparticles) enhance intestinal peptide, calcitonin, shown long-lasting decrease calcemia levels observed rats.