Regulation of lymphokine production and human T lymphocyte activation by 1,25-dihydroxyvitamin D3. Specific inhibition at the level of messenger RNA.

摘要: The steroid hormone, 1 alpha,25-dihydroxyvitamin D3 (calcitriol), has been shown to inhibit T cell proliferation, primarily through inhibition of interleukin 2 (IL-2) production. In these experiments, we show that calcitriol also markedly inhibited production the lymphokine, gamma interferon (IFN-gamma), by activated human lymphocytes. Regulation both IL-2 and IFN-gamma as well transferrin receptor (TfR) expression was apparent at messenger RNA (mRNA) level determined Northern blotting. decrease in mRNA occurred with treatment coordinate not up 12 h after phytohemagglutinin stimulation, whereas decreased accumulation TfR present before 24-36 h. Furthermore, effects on IL-2, IFN-gamma, were specific; actin comparable between control treated cells. These data indicate regulated proteins associated activation transcriptional mediated a specific, fashion.