Clinical utility of squamous and transitional nuclear structure alterations induced by Schistosoma haematobium in chronically infected adults with bladder damage verified by ultrasound in Ghana.

摘要: OBJECTIVE To evaluate the clinical utility of quantitative nuclear morphometry--i.e., alteration in size/shape, DNA content and chromatin structure-of intact cells obtained from sediment urine specimens collected people living an area highly endemic for Schistosoma haematobium Ghana. STUDY DESIGN Digital images Feulgen-DNA-stained squamous cell (SC) transitional (TC) urothelial nuclei were captured using AutoCyte imaging system, morphometric descriptors (NMDs) calculated. A total 3,495 4,523 SC TC normal bladder ultrasound subjects (n =21) 3,465 3,064 severely abnormal = 20) captured. RESULTS Univariate logistic regression analyses pooled training sets showed that 27/40 NMDs 24/40 univariately significant differentiating between SCs TCs with ultrasound. Multivariate models constructed > or 50% inclusion frequency yielded AUC-ROCs 75.23% 74.42% validation, 69.90% 66.70% validation. Further, a patient-specific model predicted severe damage AUC-ROC 86.90%, yielding sensitivity, specificity accuracy 85.00%, 76.19% 80.49%, respectively. CONCLUSION Quantitative structure alterations can be used to make noninvasive assessment cytologic changes observed both epithelia due S infection.