Dietary Suppression of the Mammary CD29hiCD24+ Epithelial Subpopulation and Its Cytokine/Chemokine Transcriptional Signatures Modifies Mammary Tumor Risk in MMTV-Wnt1 Transgenic Mice

摘要: Diet is highly linked to breast cancer risk, yet little known about its influence on mammary epithelial populations with distinct regenerative and hence, tumorigenic potential. To investigate this, we evaluated the relative frequency of lineage-negative CD29hiCD24+, CD29loCD24+ CD29hiThy1+CD24+ subpopulations in pre-neoplastic tissue adult virgin MMTV-Wnt1-transgenic mice fed either control (Casein) or soy-based diets. We found that cells exposed soy diet exhibited a lower percentage CD29hiCD24+Lin− population, decreased ability form mammospheres culture, outgrowth potential when transplanted into cleared fat pads, reduced appearance tumor-initiating cells, than those diet-fed mice. had no comparable CD29loCD24+Lin− population. Global gene expression profiling CD29hiCD24+subpopulation revealed markedly altered genes important inflammation, cytokine chemokine signaling, proliferation. Soy-fed casein-fed showed tumor incidence, shorter latency, systemic levels estradiol 17-β, progesterone interleukin-6. Our results provide evidence for functional impact specific may relate risk suggest diet-regulated cues can be further explored assessment prevention.