Mammary Stem Cells and Breast Cancer—Role of Notch Signalling

摘要: Adult stem cells are found in numerous tissues of the body and play a role tissue development, replacement repair. Evidence shows that breast multipotent can self renew, which key characteristics cells, single cell enriched with surface markers has ability to grow fully functional mammary gland vivo. Many groups have extrapolated cancer hypothesis from haematopoietic system solid cancers, where using vitro culture techniques vivo transplant models established evidence colon, pancreas, prostate, brain cancers. In report we describe for cells; studies consistently show like initiating populations be makers CD44+/CD24- upregulated genes include Notch. Notch signalling been highlighted as pathway involved development is frequently dysregulated invasive cancer. We investigated pre-invasive lesion, ductal carcinoma situ (DCIS), aberrant activation an early event High expression 1 intracellular domain (NICD) DCIS also predicted reduced time recurrence 5 years after surgery. Using non-adherent sphere technique grown mammospheres primary tissue, self-renewal capacity, measured by number mammosphere were increased normal tissue. A gamma-secretase inhibitor, DAPT, inhibits all four receptors 4 neutralising antibody shown reduce formation, indicating other pathways may represent novel therapeutic targets prevent