Human cortical glial tumors contain neural stem-like cells expressing astroglial and neuronal markers in vitro.

摘要: Neural stem cells from neurogenic regions of mammalian CNS are clonogenic in an vitro culture system exploiting serum and anchorage withdrawal medium supplemented with methyl cellulose the pleiotropic growth factors EGF, FGF2, insulin. The aim this study was to test whether cortical glial tumors contain stem-like capable, under system, forming clones showing intraclonal heterogeneity expression neural lineage-specific proteins. high frequencies clone-forming (about 0.1-10 x 10(-3)) clinical tumor specimens mutated p53, normal human CNS, suggest that ability form is induced epigenetically. RT-PCR analyses populations brain- tumor-derived sister revealed transcripts for nestin, neuron-specific enolase, fibrillary acidic protein (GFAP). However, were different derived brain specific genes associated cell fate determination via Notch-signaling pathway (Delta Jagged), survival at G2 or mitotic phases (Survivin). Moreover, individual glioma-derived immunopositive separately GFAP neuronal beta-III tubulin, as well single coexpressing both markers. data latent critical characteristics can be epigenetically by conditions not only but also tumors. genetically defective responses epigenetic stimuli may contribute gliomagenesis developmental pathological