Bioinformatics and Database Searching
作者： Surendra Dasari , David L. Tabb
DOI: 10.1002/9781118116555.CH9
关键词:
摘要: Shotgun proteomics has become the method of choice for identifying proteins in biological samples. This strategy relies on digesting into peptides and sequencing them by using tandem mass spectrometry (MS/MS). Peptides which product-ion spectra are captured then identified a database search engine. Resulting peptide identifications filtered to retain most reliable candidates. To complete this process, list protein is inferred from identifications. In chapter, we introduce basic concepts usingMS/MS.Weprovide detailed picture automated identification MyriMatch Finally, present process filtering inference IDPicker software.
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sci-hub.se 参考文章(19)
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