The course of allergen-induced leukocyte infiltration in human and experimental asthma

摘要: Background Although the timing of allergen-induced bronchoconstriction is well defined, there little information about kinetics leukocyte infiltration in asthma and its comparability between human animal models asthma. Objective To investigate systematically into airway lumen experimental by using bronchoalveolar lavage. Methods Patients with allergic were lavaged at different time points as long 1 week after segmental allergen challenge. Allergen-sensitized mice 3 weeks Differential cell counts, lymphocyte subsets, cytokines assessed lavage fluid. Results In both models, neutrophil was a relatively early event (maximum: 18 hours challenge). contrast, eosinophil peaked 42 (human model) to 4 days (mouse challenge, paralleled an IL-5 peak this period. There elevated macrophage counts over period several challenge models. Lymphocytes (predominantly CD4 + T cells) model, but not until 2 murine model. Conclusion Early accumulation (within challenge) delayed are common features inflammation, whereas dependent on Clinical implications Similarities granulocytes suggest role for these cells asthma, presumed orchestration inflammation lymphocytes appears differ