作者: Tokuichiro Seri , Keishi Matsumoto
DOI: 10.1016/S0021-9673(01)85899-4
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摘要: Abstract Eight 17-hydroxycorticosteroids and eight 17-ketosteroids were systematically separated from one another with chromatographic systems using partially esterified Amberlite IRC-50 as the stationary phase. The steroids first aqueous alcohol mobile phases (eluent A); these which only poorly resolved, if at all, then each other eluents containing a little water (eluents B C). This method was applied to analysis of urinary steroids, elution patters neutral fraction extracted β-glucuronidase hydrolysate human urine are described.