Chromatographic separation of 17-hydroxycorticosteroids and 17-ketosteroids

作者: Tokuichiro Seri , Keishi Matsumoto

DOI: 10.1016/S0021-9673(01)85899-4

关键词:

摘要: Abstract Eight 17-hydroxycorticosteroids and eight 17-ketosteroids were systematically separated from one another with chromatographic systems using partially esterified Amberlite IRC-50 as the stationary phase. The steroids first aqueous alcohol mobile phases (eluent A); these which only poorly resolved, if at all, then each other eluents containing a little water (eluents B C). This method was applied to analysis of urinary steroids, elution patters neutral fraction extracted β-glucuronidase hydrolysate human urine are described.

参考文章(11)
Tokuichiro Seki, Chromatographic separation of oestriol, oestrone and oestradiol-17 beta. Nature. ,vol. 181, pp. 768- 768 ,(1958) , 10.1038/181768A0
Basil. Vassilliou, Robert. Kunin, Fractionation of Fine Particle-Sized Ion Exchange Resins. Analytical Chemistry. ,vol. 35, pp. 1328- 1329 ,(1963) , 10.1021/AC60202A076
E. Epstein, An aqueous Zimmermann reagent for the determination of 17-ketosteroids Clinica Chimica Acta. ,vol. 7, pp. 735- 737 ,(1962) , 10.1016/0009-8981(62)90161-4
Tokuichiro Seki, Chromatographic separation of some Δ4-3-ketosteroids Journal of Chromatography A. ,vol. 2, pp. 667- 669 ,(1959) , 10.1016/S0021-9673(01)86366-4
Tokuichiro Seki, Keishi Matsumoto, Chromatographic separation of 17-ketosteroids Journal of Chromatography A. ,vol. 10, pp. 400- 404 ,(1963) , 10.1016/S0021-9673(01)92327-1
G. A. Levvy, A. J. Hay, C. A. Marsh, Properties of limpet β-glucuronidase Biochemical Journal. ,vol. 65, pp. 203- 208 ,(1957) , 10.1042/BJ0650203
TOKUICHIRO SEKI, Chromatographic Separation of Œstriol, Œstrone and Œstradiol-17β Nature. ,vol. 181, pp. 768- 768 ,(1958) , 10.1038/181768A0