Quantification of subclasses of human colonic myenteric neurons by immunoreactivity to Hu, choline acetyltransferase and nitric oxide synthase.
作者： e. m. a. murphy , d. defontgalland , m. costa , s. j. h. brookes , d. a. wattchow
DOI: 10.1111/J.1365-2982.2006.00843.X
关键词:
摘要: An accurate method to count human enteric neurons is essential develop a comprehensive account of the classes nerve cells responsible for gut function and dysfunction. The majority in nervous system utilize acetyl choline, or nitric oxide, combination these, as neurotransmitters. Antisera raised against RNA-binding protein Hu, were used identify cell bodies whole mounts myenteric plexus colon, then utilized analyse immunoreactive combinations choline acetyltransferase oxide synthase. Hu provided reliable means apparently all bodies, revealing 10% more than labelling with neuron specific enolase, no glial revealed by S100. ChAT+/NOS- accounted 48% (+/-3%) ChAT-/NOS+ 43% (+/-2.5%). ChAT+/NOS+ comprised 4% (+/-0.5) total number neurons, novel class small ChAT-/NOS- making up 5% (+/-0.9%) cells, was described first time.
flinders.edu.au
sci-hub.se 参考文章(65)
Neuroeffector Transmission in the Intestine In: Innervation of the Gastrointestinal Tract. (295-). (2003). pp. 315- 360 ,(2002) , 10.3109/9780203217016-11
Axel Brehmer, D. Ganns, W. Neuhuber, F. Schrödl, Investigation of general and cytoskeletal markers to estimate numbers and proportions of neurons in the human intestine. Histology and Histopathology. ,vol. 21, pp. 41- 51 ,(2006) , 10.14670/HH-21.41
Anne E. Bishop, Julia M. Polak, P. Facer, G.-L. Ferri, P.J. Marangos, A.G.E. Pearse, Neuron Specific Enolase: A Common Marker for the Endocrine Cells and Innervation of the Gut and Pancreas Gastroenterology. ,vol. 83, pp. 902- 915 ,(1982) , 10.1016/S0016-5085(82)80023-1
J. Dalmau, H. M. Furneaux, J. B. Posner, C. Cordon-Cardo, The expression of the Hu (paraneoplastic encephalomyelitis/sensory neuronopathy) antigen in human normal and tumor tissues American Journal of Pathology. ,vol. 141, pp. 881- 886 ,(1992)
A Belai, G Burnstock, Distribution and colocalization of nitric oxide synthase and calretinin in myenteric neurons of developing, aging, and Crohn's disease human small intestine. Digestive Diseases and Sciences. ,vol. 44, pp. 1579- 1587 ,(1999) , 10.1023/A:1026658826010
D.A. Wattchow, D.T. Cass, J.B. Furness, M. Costa, P.E. O'Brien, K.E. Little, J. Pitkin, Abnormalities of peptide-containing nerve fibers in infantile hypertrophic pyloric stenosis. Gastroenterology. ,vol. 92, pp. 443- 448 ,(1987) , 10.1016/0016-5085(87)90140-5
D.A. Wattchow, J.B. Furness, M. Costa, Distribution and coexistence of peptides in nerve fibers of the external muscle of the human gastrointestinal tract Gastroenterology. ,vol. 95, pp. 32- 41 ,(1988) , 10.1016/0016-5085(88)90287-9
Simon J.H. Brookes, Classes of enteric nerve cells in the guinea-pig small intestine. Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology. ,vol. 262, pp. 58- 70 ,(2001) , 10.1002/1097-0185(20010101)262:1<58::AID-AR1011>3.0.CO;2-V
Anthony J Porter, David A Wattchow, Simon Jh Brookes, Marcello Costa, Projections of nitric oxide synthase and vasoactive intestinal polypeptide-reactive submucosal neurons in the human colon. Journal of Gastroenterology and Hepatology. ,vol. 14, pp. 1180- 1187 ,(1999) , 10.1046/J.1440-1746.1999.02026.X
R.R. de Souza, H.B. Moratelli, N. Borges, E.A. Liberti, Age-induced nerve cell loss in the myenteric plexus of the small intestine in man. Gerontology. ,vol. 39, pp. 183- 188 ,(1993) , 10.1159/000213532