Osteocalcin cluster: Implications for functional studies
作者： Christelle Desbois , Gerard Karsenty
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摘要: Osteocalcin is a skeletal member of the family extracellular mineral binding Gla protein. synthesized only by osteoblast and it secreted into bone matrix at time mineralization. The properties osteocalcin as well its spatial temporal pattern expression suggest that plays role during mineralization, however until now biological function unclear. To understand skeletogenesis we mutated two genes homologous recombination in embryonic stem (ES) cells. Eight targeted clones were identified Southern analysis using external probes. One these contributed to germ line mouse chimera. Interbreeding heterozygotes currently progress. Mutant mice will be useful vivo.
sci-hub.se 参考文章(16)
R M Pauli, D F Mosher, J W Suttie, J B Lian, Association of congenital deficiency of multiple vitamin K-dependent coagulation factors and the phenotype of the warfarin embryopathy: clues to the mechanism of teratogenicity of coumarin derivatives. American Journal of Human Genetics. ,vol. 41, pp. 566- 583 ,(1987)
C. Desbois, D.A. Hogue, G. Karsenty, The mouse osteocalcin gene cluster contains three genes with two separate spatial and temporal patterns of expression. Journal of Biological Chemistry. ,vol. 269, pp. 1183- 1190 ,(1994) , 10.1016/S0021-9258(17)42240-X
J.W. Poser, P.A. Price, A method for decarboxylation of gamma-carboxyglutamic acid in proteins. Properties of the decarboxylated gamma-carboxyglutamic acid protein from calf bone. Journal of Biological Chemistry. ,vol. 254, pp. 431- 436 ,(1979) , 10.1016/S0021-9258(17)37935-8
Philipps Soriano, Charles Montgomery, Robert Geske, Allan Bradley, Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice Cell. ,vol. 64, pp. 693- 702 ,(1991) , 10.1016/0092-8674(91)90499-O
J. Lian, C. Stewart, E. Puchacz, S. Mackowiak, V. Shalhoub, D. Collart, G. Zambetti, G. Stein, Structure of the rat osteocalcin gene and regulation of vitamin D-dependent expression. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 1143- 1147 ,(1989) , 10.1073/PNAS.86.4.1143
Nadine M. Ritter, Mary C. Farach-Carson, William T. Butler, Evidence for the formation of a complex between osteopontin and osteocalcin. Journal of Bone and Mineral Research. ,vol. 7, pp. 877- 885 ,(2009) , 10.1002/JBMR.5650070804
P C Comp, R R Nixon, M R Cooper, C T Esmon, FAMILIAL PROTEIN S DEFICIENCY IS ASSOCIATED WITH RECURRENT THROMBOSIS Journal of Clinical Investigation. ,vol. 74, pp. 2082- 2088 ,(1984) , 10.1172/JCI111632
Bruce Furie, Barbara C. Furie, The molecular basis of blood coagulation Cell. ,vol. 53, pp. 505- 518 ,(1988) , 10.1016/0092-8674(88)90567-3
D. L. Diuguid, M. J. Rabiet, B. C. Furie, H. A. Liebman, B. Furie, Molecular basis of hemophilia B: a defective enzyme due to an unprocessed propeptide is caused by a point mutation in the factor IX precursor Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 5803- 5807 ,(1986) , 10.1073/PNAS.83.16.5803
Elizabeth J. Robertson, Pluripotential stem cell lines as a route into the mouse germ line Trends in Genetics. ,vol. 2, pp. 9- 13 ,(1986) , 10.1016/0168-9525(86)90161-7