Sperm Protamine-Status Correlates to the Fertility of Breeding Bulls

摘要: During fertilization, spermatozoa make essential contributions to embryo development by providing oocyte activating factors, centrosomal components, and paternal chromosomes. Protamines are for proper packaging of sperm DNA; however, in contrast the studies oocyte-related female infertility, influence chromatin structure on male infertility has not been evaluated extensively. The objective this study was determine content bull evaluating DNA fragmentation, maturity/protamination, PRM1 protein status, nuclear shape from bulls with different fertility. Relationships between protamine 1 (PRM1) integrity were ascertained Holstein varied (high vs. low) but acceptable Sperm fragmentation maturity (protamination) tested using Halomax assay toluidine blue staining, respectively. assayed Western blotting in-gel densitometry, flow cytometry, immunocytochemistry. Fragmentation increased significantly reduced low-fertility compared those high-fertility bulls. Field fertility scores negatively correlated percentage displaying protamination fragmented Halomax, Bull also positively cytometry. However, detection alone predictive In immunocytochemistry, abnormal showed either a lack or scattered localization apical/acrosomal region nuclei. distorted conclusion, we that inadequate amount associated defects structure, coinciding These findings highly significant because they reveal molecular morphological phenotypes mammalian