The antipsychotic drugs olanzapine and haloperidol modify network connectivity and spontaneous activity of neural networks in vitro.

摘要: Impaired neural synchronization is a hallmark of psychotic conditions such as schizophrenia. It has been proposed that schizophrenia-related cognitive deficits are caused by an unbalance reciprocal inhibitory and stimulatory signaling. This supposedly leads to decreased power induced gamma oscillations during the performance tasks. In light this hypothesis efficient antipsychotic treatment should modify connectivity local circuits. To address issue, we investigated model hippocampal neuronal networks in vitro. Inhibitory excitatory innervation GABAergic glutamatergic neurons was quantified using immunocytochemical markers automated routine estimate network connectivity. The first generation (FGA) second (SGA) drugs haloperidol olanzapine, respectively, differentially modified density synaptic inputs. Based on observed synapse modifications, developed computational reliably predicted distinct changes activity patterns. results modeling were confirmed spontaneous measurements multiple electrode array (MEA) technique. When cultures treated with overall increased, whereas had opposite effect. We conclude FGAs SGAs affect balance between inhibition excitation networks.