A high-throughput method for measuring growth and loss rates in microalgal cultures
作者： Birger Skjelbred , Bente Edvardsen , Tom Andersen
DOI: 10.1007/S10811-012-9819-Z
关键词:
摘要: A miniaturized and low-cost assay for algal growth loss rates, estimation of compensation light was developed optimized. Microalgal cultures were grown in white 96-well microplates to estimate specific rates at six temperatures, five salinities eight levels. Data from black 24-well conditions used addition light. Absorption reflection different the microplates. Growth estimated daily vivo fluorescence (IVF) measurements using a microplate reader fitted with fluorometer. To validate assay, IVF compared cell counting by flow cytometry. Maximal rate test alga Pseudochattonella farcimen (Heterokonta) 0.52 ± 0.05 day−1 optimal temperatures ranging 9 14°C 18–26 psu. Lowest value as photosynthetic photon flux density (PPFD) 4.2 ± 1.2 μmol photons m−2 s−1, lowest saturation light, 34.1 ± 3.7 μmol observed temperature range 5–11°C salinity 23–28 Minimum obtained 5–8°C 26–31 Blooms P. have been recorded nature under similar those minimizing rather than maximizing this study. The microalgal described here allows large number be tested, accurate obtained.
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