Activation of proliferation and differentiation of dental follicle stem cells (DFSCs) by heat stress.

摘要: Objectives Adult stem cells (ASCs) remain in a slowly cycling/quiescent state under normal physiological conditions, but they can be awakened from this by certain factors, such as injury signals. Previously, our group has shown that dental follicle (DFSCs) appear to proliferate more rapidly than their non-stem cell counterparts at elevated temperatures. The study described here aimed (i) elucidate optimal temperature which culture DFSCs, (ii) determine whether temperatures could enhance differentiation capability of DFSCs and (iii) characterize osteogenic marker expression temperatures. Materials methods DFSCs obtained rat first molars were cultured 37 (control), 38, 39, 40 41 oC. Cell proliferation was evaluated Alamar blue reduction assay mean numbers viable dissociated cells. Osteogenic after 7 or 14 days induction. Expression selected genes also assessed during the cells. Results Increased seen heat-stress 38o, 39o 40 oC. revealed maximal osteogenesis when 39 Moreover, some osteogensis-associated markers had conditions. Conclusions Under determined increased proliferation, genes. Thus, it is likely serve factor activate adult