Ex vivo bone morphogenetic protein 2 gene delivery using periodontal ligament stem cells for enhanced re-osseointegration in the regenerative treatment of peri-implantitis.
作者： Shin-Young Park , Kyoung-Hwa Kim , Eun-Hye Gwak , Sang-Hoon Rhee , Jeong-Cheol Lee
DOI: 10.1002/JBM.A.35145
关键词:
摘要: Peri-implantitis is a chronic inflammatory process with advanced bone loss and impaired healing potential. For peri-implantitis treatment, tissue engineering can be applied to enhance regeneration of peri-implant defects. This study aimed evaluate ex vivo morphogenetic protein 2 (BMP2) gene delivery using canine periodontal ligament stem cells (PDLSCs) for Canine PDLSCs were transduced adenoviral vectors containing BMP2 (BMP2/PDLSCs). After was induced by ligature placement in six beagle dogs, regenerative procedures performed; hydroxyapatite (HA) particles collagen gel autologous (PDLSC group) or BMP2/PDLSCs (BMP/PDLSC without (control grafted into the defects covered an absorbable membrane. Three months later, animals sacrificed. In vitro, showed similar levels cell properties PDLSCs, such as colony-forming efficiency expression MSC markers STRO-1 CD 146. produced BMP-2 until day 21 at concentration 4-8 ng/mL. vivo, BMP2/PDLSC group significantly more new formation re-osseointegration compared other groups. conclusion, enhanced
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