INTERACTION OF DIBUCAINEHCl LOCAL ANESTHETICS WITH BACTERIORHODOPSIN IN PURPLE MEMBRANE: A SPECTROSCOPIC STUDY
作者： C. T. LIN , Y. G. CHYAN , G. C. KRESHECK , HERBERT C. BITTING JR , M. A. EL-SAYED
DOI: 10.1111/J.1751-1097.1989.TB08436.X
关键词:
摘要: Several spectroscopic techniques (absorption, emission, transient absorption and differential scanning calorimetry--DSC) were used to investigate the deprotonation of dibucaine.HCl in a hydrophobic environment, interaction sites mechanisms local anesthetic on bacteriorhodopsin (bR) purple membrane. The important results are summarized as follows: (1) visible features native (lambda max = 568 nm) deionized 608 bR sensitive amount added; (2) emission spectrum embedded retinal-free mutant is similar that dibucaine free base Triton X-100 micellar solutions; (3) phosphorescence at 77 K completely quenched by fluorescence quenching rate for incorporated was determined kq 4.09 x 10(13) M-1 s-1; (4) incorporation inhibits slow component formation M412 decreases photochemical cycle bR; (5) thermal stability measured DSC presence absence yielded an endothermic transition 95.9 +/- 1.0 degrees C with 13.6 J/g (3.25 0.12 cal/g) enthalpy changes. All observations suggest action site anesthetic, dibucaine.HCl, near or chromophore, i.e. retinal Schiff bR. membrane probably via specific binding, but not conformational mechanism.
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