Quality of cryopreserved African catfish sperm following post-thaw storage

摘要: Summary Experiments were carried out to test the fertilizing capacity and viability of cryopreserved African catfish (Clarias gariepinus) sperm following post-thaw storage at 4°C activation with water. In first experiment, was thawed stored in a refrigerator for time periods ranging from 0 96 h before being used fertilization. all cases 24 h resulted highest fertilization percentages (37 ± 9%) which significantly higher than that observed immediately after thawing (21 ± 4%, P < 0.05). These observations later confirmed by flow cytometric assessment membrane integrity spermatozoa showed an increase percentage membrane-intact cells 2 h (66 ± 3%) 26 h (77 ± 3%). second fresh activated water different post-activation 120 s. The rate (72 ± 12%) when eggs fertilized 20 s, however, 120 s still able fertilize eggs, albeit low (2 ± 3%). It also noted trial experiment 2 freshly extracted semen (P < 0.05).