Application of Luminex Gastrointestinal Pathogen Panel to human stool samples from Côte d'Ivoire.

摘要: Introduction: Gastrointestinal infections caused by viruses, bacteria, and parasites are endemic in most developing countries due to inadequate provision of safe water supplies, sanitation, hygiene. To investigate the enteric pathogens infecting people living Cote d’Ivoire, Luminex Pathogen Panel (xTAG GPP) assay was used analyze 34 human fecal samples. This study represents first application this technology samples from a sub-Saharan African country. Methodology: Thirty-four stool asymptomatic symptomatic patients, 1–15 years age, were analyzed xTAG GPP. The qualitative bead-based multiplexed molecular diagnostic test able identify concurrently 15 pathogens, including parasites. Results: Overall, 22 out (64.7%) specimens detected be positive Sixteen subjects, 10 patients (45.4%) showed co-infections. G. duodenalis both mono- co-infections, representing frequent pathogen, followed enterotoxigenic Escherichia coli (ETEC) LT/ST. Four norovirus isolates also assigned genogroups I II. Conclusions: Considering burden countries, particularly among children, high rate co-infections shows need for tools such as GPP improve diagnosis treatment these areas.