[54] In vivo oxidative DNA damage : measurement of 8-hydroxy-2'-deoxyguanosine in DNA and urine by high-performance liquid chromatography with electrochemical detection

摘要: Abstract HPLC with electrochemical detection is a highly sensitive and selective method for detecting the oxidatively modified DNA residue oh 8 dG. By this method, of dG from urine offers powerful approach assessing in vivo oxidative damage. Application technique to permits quantitation steady-state levels deoxynucleoside overcomes problems associated extremely low present DNA. In addition, selectivity gained by eliminates problem separating signal normal deoxynucleosides. The gdG complements measurement estimating cumulative damage body. urinary assay provides noninvasive means measuring type laboratory animals human populations. Thus, an individual animal or subject may be monitored over time, possibly under various prooxidant conditions, using as marker This analytical allow one estimate exposure conditions lifestule, genetic predisposition, degenerative diseases, environmental toxins.