In Situ DESI-MSI Lipidomic Profiles of Breast Cancer Molecular Subtypes and Precursor Lesions

摘要: Clinically meaningful molecular subtypes for classification of breast cancers have been established, however, initiation and progression these remain poorly understood. The recent development desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) facilitates the convergence analytical chemistry traditional pathology, allowing chemical profiling with minimal tissue pretreatment in frozen samples. Here, we characterized composition cancer DESI-MSI. Regions interest were identified, including invasive (IBC), ductal carcinoma situ (DCIS), adjacent benign (ABT), metabolomic profiles at 200 μm elaborated using Biomap software Lasso method. Top ions identified IBC regions included polyunsaturated fatty acids, deprotonated glycerophospholipids, sphingolipids. Highly saturated lipids, as well antioxidant molecules [taurine (m/z 124.0068), uric acid 167.0210), ascorbic 175.0241), glutathione 306.0765)], able to distinguish from ABT. Moreover, luminal B triple-negative showed more complex lipid compared A HER2 subtypes. DCIS distinguished on basis cell signaling apoptosis-related [fatty acids (341.2100 382.3736 m/z) glycerophospholipids (PE (P-16:0/22:6, m/z 746.5099, PS (38:3), 812.5440)]. In summary, DESI-MSI distinct between across cancer, potential implications pathogenesis. SIGNIFICANCE: These findings present first four DCIS, normal tissue, add understanding their