The effect of anti-beta 43-47 Fab fragments on expression of the polymerization sites in the E domain of fibrinogen.

摘要: In order to assess the significance of aminoterminal residues B beta chain in expression polymerization sites E domain, we have prepared polyclonal antiserum against a synthetic peptide corresponding 43-47 human fibrinogen. Affinity purified immunoglobulin IgG and Fab from these antibodies reacted strongly specifically with pentapeptide intact fibrinogen molecule. This specificity was determined both by radioimmunoassay Western blot analysis its plasmic fragments described our previous paper (Cierniewski et al., 1986, Biochim. Biophys. Acta, 884, 594-597). Immunochemically anti their were strong inhibitors fibrin monomer polymerization. Our results imply that amino acid sequence recognized may be close vicinity contact domain.