NCL‐CB11, a new monoclonal antibody recognizing the internal domain of the c‐erbB‐2 oncogene protein effective for use on formalin‐fixed, paraffin‐embedded tissue
作者： Ian P. Corbett , James A. Henry , Brian Angus , Caroline J. Watchorn , Linda Wilkinson
关键词:
摘要: The c-erbB-2 proto-oncogene encodes a 190 k Mr protein representing putative growth factor receptor with considerable homology to the EGF receptor. Gene amplification and overexpression of oncogene have been demonstrated in variety tumours including breast cancer, where it is associated poor prognosis. In this study we produced characterized mouse monoclonal antibody, designated NCL-CB11, protein. NCL-CB11 was generated synthetic peptide sequence corresponding site predicted antigenicity near C-terminus internal domain produces intense membrane-associated immunohistochemical staining proportion human cancer cells. specificity antibody supported by Western blotting immunoprecipitation studies. Reactivity an confirmed necessity cell permeabilization for reactivity fluorescence-activated sorter (FACS) analysis. A high degree correlation between using gene has observed. should prove be valuable reagent investigations into pathological significance expression.
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