Enantioseparation of new nucleoside analogs, related to d4T and acyclovir, by chiral capillary electrophoresis using highly sulfated β‐cyclodextrins

摘要: Baseline separation of some new acyclic nucleosides which are potential antiviral agents was achieved using cyclodextrin capillary zone electrophoresis (CD-CZE). A method for the enantiomeric resolution these compounds and determination their purity developed anionic CDs (highly sulfated-CD or highly S-CD) as chiral selectors capillaries, were dynamically coated with polyethylene oxide (PEO). Operational parameters including (i) nature concentration selectors, (ii) organic modifiers, (iii) temperature, (iv) applied voltage investigated. The use charged provides a supplementary driving force in running buffer by inclusion CD cavity. S-CD found to be most effective complexing agent allowed good resolution. complete five nucleoside analogs obtained 25 mM phosphate buffer, pH 2.5, containing either S-alpha-CD, S-beta-CD S-gamma-CD at 30 degrees C an field 0.30 kV/cm. apparent association constants complexes calculated. enantiomer migration order molecules investigated determined detection limit impurities vary between 0.34 3.56 ng.mL(-1) first enantiomer.