Immunohistochemical analysis of the rat central nervous system during experimental allergic encephalomyelitis, with special reference to Ia-positive cells with dendritic morphology.

摘要: The rat central nervous system (CNS) during experimental allergic encephalomyelitis (EAE) was analyzed immunohistochemically from the preclinical to recovery stage by using monoclonal antibodies specific for T lymphocyte subsets and Ia antigen. Through combination of avidin-biotin technique carefully selected fixative, cells with dendritic morphology (DC) infiltrating mononuclear were clearly intensely demonstrated in CNS parenchyma EAE. In normal complete Freund's adjuvant (CFA)-injected controls, there no inflammatory foci. (OX3)-positive parenchymal not detected, whereas W3/25 stained DC that located mainly white matter W3/13 axons. At stage, 11 days after CNS/CFA sensitization, a few clusters Ia+ detected some sections spinal cord. number increased as clinical signs developed (P less than 0.001). rats score 1 or 2, perivascular region closely associated lymphocytes. However, at moribund state (score 3), evenly distributed gray on almost all recovered rats, numbers foci those EAE Rats without throughout course also contained DC. Double immunofluorescent staining OX3 anti-glial fibrillary acidic protein (GFAP) antiserum negative GFAP. Their distribution similar nucleoside diphosphatase-positive cells, suggesting are microglia. contrast DC, astrocytes endothelial express detectable levels antigen control rats. These findings suggest brain other may be involved local immune interaction. play significant role presentation