作者: Yong Zhu , Dongteng Liu , Zoe C. Shaner , Shixi Chen , Wanshu Hong
关键词:
摘要: Progestins, progesterone derivatives, are the most critical signaling steroid for initiating final oocyte maturation (FOM) and ovulation, in order to advance fully-grown immature oocytes become fertilizable eggs basal vertebrates. It is well-established that progestin induces FOM via an elusive membrane receptor a nongenomic process, which precedes triggered ovulation mediated through nuclear (Pgr) genomic pathway. To determine whether Pgr plays role mechanism during FOM, we knocked out zebrafish using transcription activator-like effector nucleases (TALENs) studied phenotypes of knockouts (Pgr-KOs). Three TALENs-induced mutant lines with different frame shift mutations were generated. Homozygous Pgr-KO female fish all infertile while no fertility effects evident homozygous males. Oocytes developed underwent normally vivo compared wildtype controls, but these mature trapped within follicular cells failed ovulate from ovaries. These also normal germinal vesicle breakdown (GVBD) vitro, even after treatment human chronic gonadotropin (HCG) or (17alpha,20beta-dihydroxyprogesterone DHP), typically induce oocytes. The results indicate anovulation infertility was, at least part, due lack functional Pgr-mediated adjacent Our study supports previous demonstrate steroid-dependent pathways leading first convincing evidence not essential triggering meiosis resumption.