Post-irradiation regeneration of early B-lymphocyte precursor cells in mouse bone marrow.

摘要: To examine the sequential development of early B-cell precursors in mouse bone marrow, B-lineage cells have been examined during a wave post-irradiation regeneration. Cell phenotypes defined using double-immunofluorescence labelling techniques for (i) terminal deoxynucleotidyl transferase (TdT); (ii) B220 glycoprotein, detected by binding mAb 14.8; (iii); mu heavy chains cytoplasm (c mu) and at cell surface (s mu). Three populations mu- (TdT+14.8-; TdT+14.8+; TdT-14.8+) proposed to be which would give rise c mu+s pre-B thus s mu+ B lymphocytes. From 3 7 days after sublethal dose (150 rads) whole body gamma-irradiation, recovered rapidly exceed normal numbers. The increased peaks 1.8-2.5 times numbers, preceding 1 day comparable increase overshoot cells, followed recovery TdT+14.8- peaked first 5 days, subsiding again 7-10 with shift from large- medium-sized cells. TdT+14.8+ showed later peak (6 days), more sustained numbers delayed size. substantial population 14.8+ reached maximal observed values still maintained predominantly large size profile 10 days. timing, cell-size shifts progressive amplification waves regeneration accord dynamic model TdT+14.8-,TdT+14.8+ TdT-14.8+ form three successive stages differentiation before expression chains, presumptively including stage chain gene rearrangement. In addition, results provide an experimental system enrichment marrow.