Anaerobic Oxidation of Methane at a Marine Methane Seep in a Forearc Sediment Basin off Sumatra, Indian Ocean

摘要: A cold methane-seep was discovered in a forearc sediment basin off the island Sumatra, exhibiting adapted microbial community. defined seep centre of activity, like mud volcanoes, not discovered. The area rather characterized by patchy distribution active spots. relevance AOM reflected 13C depleted isotopic signatures dissolved inorganic carbon (DIC). anaerobic conversion methane to CO2 confirmed 13C-labelling experiment. Methane fuelled vital and invertebrate community which cell numbers up 4 x 109 cells cm 3 guts crabs populating area. analysed total counting, catalyzed reporter deposition – fluorescence situ hybridisation (CARD-FISH), quantitative real-time PCR (qPCR) denaturing gradient gel electrophoresis (DGGE). CARD-FISH counts qPCR measurements showed presence Bacteria Archaea, but only small Eukarya. archaeal comprised largely members ANME-1 ANME-2. Furthermore, Crenarchaeota were frequently detected DGGE analysis. Three major bacterial phylogenetic groups (δ-Proteobacteria, candidate division OP9 Anaerolineaceae) abundant across study Several these sequences closely related genus Desulfococcus family Desulfobacteraceae, is good agreement with previously described sites. In conclusion, majority at consisted microorganisms, while higher hydrocarbons as substrates negligible.