Metagenomic analysis of microbial consortium from natural crude oil that seeps into the marine ecosystem offshore Southern California

摘要: Crude oils can be major contaminants of the marine ecosystem and microorganisms play a significant role in degradation its main constituents. To increase our understanding microbial hydrocarbon process ecosystem, we collected crude oil from an active seep area located Santa Barbara Channel (SBC) generated total about 52 Gb raw metagenomic sequence data. The assembled data comprised ∼500 Mb, representing ∼1.1 million genes derived primarily chemolithoautotrophic bacteria. Members Oceanospirillales, bacterial order belonging to Deltaproteobacteria, recruited less than 2% within SBC metagenome. In contrast, community associated with plume that developed aftermath Deepwater Horizon (DWH) blowout 2010, was dominated by which more 60% DWH plume. This suggests Oceanospirillales might microbially mediated conversion compared oil. We hypothesize this difference results being mostly anaerobic, while is aerobic. Within Archaea, phylum Euryarchaeota, 95% archaeal sequences metagenome, 50% assigned members orders Methanomicrobiales Methanosarcinales. These contain organisms capable anaerobic methanogenesis methane oxidation (AOM) these - their metabolic capabilities may fundamental ecology seep.